In this study, five measures of linkage disequilibrium, Lewontin's D', the correlation coefficient R2 and three volume measures, Dvol, Mvol and Hvol, are studied on data set comprised of bialleic markers. From simulation of marker pairs with only one allele having the allele frequency less than 0.2 in each marker, we also found that Dvol is a more reliable measure than D' when a locus contains a rare allele frequency. The data set applied in this study contains eleven isolated populations and one Caucasian outbred population. We found that the patterns of linkage disequilibrium among these twelve populations show a similarity. Three Finnish isolated populations show higher LD than others and Azores population shows the least LD. The highlight areas with the higher levels of LD are notable at position 25-32 MB and 34-43 MB. These two areas may suggest lower recombination rate.